Development of the Korean construction job exposure matrix (KoConJEM) based on experts' judgment using the 60 consolidated occupations for construction workers.

BACKGROUND: This study was conducted as an effort to develop a Korean construction job exposure matrix (KoConJEM) based on 60 occupations recently consolidated by the construction workers mutual aid association for use by the construction industry. METHODS: The probability, intensity, and prevalence of exposure to 26 hazardous agents for 60 consolidated occupations were evaluated as binary (Yes/No) or four categories (1 to 4) by 30 industrial hygiene experts. The score for risk was calculated by multiplying the exposure intensity by the prevalence of exposure. Fleiss' kappa for each hazardous agent and occupation was used to determine agreement among the 30 experts. The JEM was expressed on a heatmap and a web-based dashboard to facilitate comparison of factors affecting exposure according to each occupation and hazardous agent. RESULTS: Awkward posture, heat/cold, heavy lifting, and noise were hazardous agents regarded as exposure is probable by at least one or more experts in all occupations, while exposure to asphalt fumes was considered hazardous in the smallest number of occupations (n = 5). Based on the degree of agreement among experts, more than half of the harmful factors and most occupations showed fair to good results. The highest risk value was 16 for awkward posture for most occupations other than safety officer. CONCLUSIONS: The KoConJEM provides information on the probability, intensity, and prevalence of exposure to harmful factors, including most occupations employing construction workers; therefore, it may be useful in the conduct of epidemiological studies on assessment of health risk for construction workers.

Field demonstration for the solvent-based sampling method to perform compound-specific isotope analysis on gas-phase VOC.

The solvent-based sampling method for collecting gas-phase volatile organic compounds (VOCs) and conducting compound-specific isotope analysis (CSIA) was deployed during a controlled field study. The solvent-based method used methanol as a sink to accumulate petroleum hydrocarbons during the sampling of soil air and effluent gas. For each gaseous sample collected, carbon isotope analysis (δ13C) was conducted for a selection of five VOCs (benzene, toluene, o-xylene, cyclopentane and octane) emitted by a synthetic hydrocarbon source emplaced in the subsurface. The δ13C values obtained for gaseous VOCs (collected from soil gas and effluent gas) were compared to measurements obtained for the same VOCs present in the source material (none aqueous phase liquid - NAPL) and dissolved in groundwater to evaluate the reliability of the solvent-based sampling method in providing accurate isotope measurements. Since the NAPL source was composed of only 12 VOCs, potential bias related to the analytical procedure (such as co-elution) were avoided, hence emphasizing on field-related bias. This field evaluation demonstrated the capacity of the solvent-based method to produce precise and accurate δ13C measurements. The isotopic discrepancies between the gaseous and the NAPL values were < 1 ‰ for 39 out of the 41 comparison points, thus deemed not statistically different based on a common isotopic uncertainty error of ±0.5 ‰. Moreover, the current field study is the first field study to report δ13C measurements for up to five gas-phase VOCs obtained from the same sample, which appears to be of interest for VOC fate or forensic studies. The possibility to use several VOC isotopic measurements enabled by the sampling method would contribute to strengthen the connection assessment between gaseous VOCs and the suspected emitting source. Accordingly, the field results presented herein support the application of this sampling methodology to conduct CSIA assessment in the frame of VOC vapor studies.

Residual hydrocarbons in long-term contaminated soils: implications to risk-based management.

Petroleum hydrocarbon (PHC) contamination is a widespread and severe environmental issue affecting many countries' resource sectors. PHCs are mixtures of hydrocarbon compounds with varying molar masses that naturally attenuate at different rates. Lighter fractions attenuate first, followed by medium-molar-mass constituents, while larger molecules remain for longer periods. This results in significant regulatory challenges concerning residual hydrocarbons in long-term contaminated soils. This study examined the potential risks associated with residual PHC and its implications for risk-based management of heavily contaminated soils (23,000-26,000 mg PHC/kg). Ecotoxicological properties, such as seedling emergence and growth of two native plant species-small Flinders grass (Iseilema membranaceum) and ruby saltbush (Enchylaena tomentosa)-and earthworm survival tests in PHC-contaminated soils, were assessed. Additionally, the effects of aging on the attenuation of PHC in contaminated soils were evaluated. Toxicity responses of plant growth parameters were determined as no-observed-effect concentrations: 75%-100% for seedling emergence, < 25%-75% for plant shoot height, and 75%-100% for earthworm survival. After 42 weeks of aging, the total PHC levels in weathered soils decreased by 14% to 30% and by 67% in diesel-spiked soil due to natural attenuation. Dehydrogenase enzyme activity in soils increased during the initial aging period. Furthermore, a clear shift of bacterial communities was observed in the soils following aging, including enrichment of PHC-resistant and -utilizing bacteria-for example, Nocardia sp. This study underscores the potential of natural attenuation for eco-friendly and cost-effective soil management, underlining that its success depends on site-specific factors like water content and nutrient availability. Therefore, we recommend detailed soil assessments to evaluate these conditions prior to adopting a risk-based management approach.

Hydrocarbon adsorption mechanism of modern automobile engines and methods of reducing hydrocarbon emissions during cold start process: A review.

With the global emphasis on environmental protection and increasingly stringent emission regulations for internal combustion engines, there is an urgent need to overcome the problem of large hydrocarbon (HC) emissions caused by unstable engine cold starts. Synergistic engine pre-treatment (reducing hydrocarbon production) as well as after-treatment devices (adsorbing and oxidizing hydrocarbons) is the fundamental solution to emissions. In this paper, the improvement of hydrocarbon emissions is summarized from two aspects: pre-treatment and after-treatment. The pre-treatment for engine cold start mainly focuses on summarizing the intake control, fuel, and engine timing parameters. The after-treatment mainly focuses on summarizing different types of adsorbents and modifications (mainly including different molecular sieve structures and sizes, preparation conditions, silicon aluminum ratio, ion exchange modification, and heterogeneity, etc.), adsorptive catalysts (mainly including optimization of catalytic performance and structure), and catalytic devices (mainly including coupling with thermal management equipment and HC trap devices). In this paper, a SWOT (strength, weakness, opportunity, and threat) analysis of pre-treatment and after-treatment measures is conducted. Researchers can obtain relevant research results and seek new research directions and approaches for controlling cold start HC emissions.

The content and composition of organic compounds in the bottom sediments of the Norilsk-Pyasina water system one year after the accidental spill of diesel fuel.

One year after the emergency diesel fuel spill in Norilsk, hydrocarbon concentrations in bottom sediments of the Norilsk-Pyasina water system decreased. However the average concentrations of hydrocarbons in surface sediments decreased in the same sequence (µg/g) as in 2020: the mouth of the Ambarnaya R. (835, σ = 1788) > Bezymyanny Cr.-the Daldykan R.-the Ambarnaya R. (306, σ = 273) > the Pyasina R. (23, σ = 20) > the Pyasino Lake (12, σ = 8). Concentrations decreased due to degradation of low molecular weight hydrocarbons. The content of polycyclic aromatic hydrocarbons in 2021 also changed in a smaller range (0-1027 ng/g) than in 2020 (0-3865 ng/g). Petroleum origin of polycyclic aromatic hydrocarbons in the sediments of the Ambarnaya R. (including the mouth), Bezymyanny Cr. and the Daldykan R. is confirmed by the dominance of alkylated naphthalene homologues in their composition. Hydrocarbons accumulation in some layers of the sedimentary column is caused not only by the spill of diesel fuel, but also by the organic matter from the surrounding swamps, from wetlands and floodplain lakes, as well as by the burial of the surface layer by the 2021 flood.

Molecular insights into the microbial degradation of sediment-derived DOM in a macrophyte-dominated lake under aerobic and hypoxic conditions.

The mineralization of dissolved organic matter (DOM) in sediments is an important factor leading to the eutrophication of macrophyte-dominated lakes. However, the changes in the molecular characteristics of sediment-derived DOM during microbial degradation in macrophyte-dominated lakes are not well understood. In this study, the microbial degradation process of sediment-derived DOM in Lake Caohai under aerobic and hypoxic conditions was investigated using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and metagenomics. The results revealed that the microbial degradation of sediment-derived DOM in macrophyte-dominated lakes was more intense under aerobic conditions. The microorganisms mainly metabolized the protein-like substances in the macrophyte-dominated lakes, and the carbohydrate-active enzyme genes and protein/lipid-like degradation genes played key roles in sediment-derived DOM degradation. Organic compounds with high H/C ratios such as lipids, carbohydrates, and protein/lipid-like compounds were preferentially removed by microorganisms during microbial degradation. Meanwhile, there was an increase in the abundance of organic molecular formula with a high aromaticity such as tannins and unsaturated hydrocarbons with low molecular weight and low double bond equivalent. In addition, aerobic/hypoxic environments can alter microbial metabolic pathways of sediment-derived DOM by affecting the relative abundance of microbial communities (e.g., Gemmatimonadetes and Acidobacteria) and functional genes (e.g., ABC.PE.P1 and ABC.PE.P) in macrophyte-dominated lakes. The abundances of lipids, unsaturated hydrocarbons, and protein compounds in aerobic environments decreased by 58 %, 50 %, and 44 %, respectively, compared to in hypoxic environments under microbial degradation. The results of this study deepen our understanding of DOM biodegradation in macrophyte-dominated lakes under different redox environments and provide new insights into nutrients releases from sediment and continuing eutrophication in macrophyte-dominated lakes.

Influences of extracellular polymeric substances (EPS) recovered from waste sludge on the ability of Jiaozhou Bay to self-remediate of diesel-polluted seawater.

The introduction of EPS recovered from waste sludge may have an impact on the process of microbial remediation of oil-contaminated seawater. This study investigated the effect of EPS on the self-remediation capacity of diesel-polluted seawater in Jiaozhou Bay. Hydrocarbon attenuation and microbial activity were monitored in seawater collected from five islands after diesel and N, P addition, with and without EPS, incubated under aerobic conditions. Compared to seawater without EPS, degradation of TPH (total petroleum hydrocarbon) doubled and improved degradation of non-volatile (C16-C24) hydrocarbons to some extent in EPS-added seawater. The introduction of EPS led to changes in microbiota richness and diversity, significantly stimulating the growth of Proteobacteria and Firmicutes phyla or Bacillus and Pseudomonas genera. RT-qPCR analysis indicated EPS caused higher increases in cytochrome P450 gene copies than alkB. Prediction of alkane decay genes from 16S rRNA sequencing data revealed that EPS addition obviously promoted genes related to ethanol dehydrogenation function in the microbial community. Additionally, EPS enhanced the enzymatic activities of alkane hydroxylase, ethanol dehydrogenase, phosphatase and lipase, but increased protease and catalase inconspicuously. The above outlook that environmental sustainability of EPS from waste sludge for diesel-contaminated seawater remediation may provide new perspectives for oil spill bioremediation.

Advanced separation of mineral oil aromatic hydrocarbons by number of aromatic rings using donor-acceptor-complex chromatography to extend on-line coupled liquid chromatography-gas chromatography.

An automated implementation for a subfractionation of mineral oil aromatic hydrocarbons (MOAH) into a mono-/di-aromatic fraction (MDAF) and a tri-/poly-aromatic fraction (TPAF) is presented, which is highly demanded by the European Food Safety Authority (EFSA) respecting the genotoxic and carcinogenic potential of MOAH. For this, donor-acceptor-complex chromatography (DACC) was used as a selective stationary phase to extend the conventional instrumental setup for the analysis of mineral oil hydrocarbons via on-line coupled liquid chromatography-gas chromatography-flame ionization detection (LC-GC-FID). A set of six new internal standards was introduced for the verification of the MOAH fractionation and a quantification of MDAF and TPAF, respectively. The automated DACC approach was applied to representative petrochemical references as well as to food samples, such as rice and infant formula, generally showing well conformity with results obtained by state-of-the-art analysis using two-dimensional GC (GCxGC). Relative deviations of DACC/LC-GC-FID compared to GCxGC-FID methods regarding the ≥ 3 ring MOAH content ranged between -50 and +6 % (median: -2 %, all samples, only values above limit of quantification). However, crucial deviations mainly result from "border-crossing" substances, e.g., dibenzothiophenes or partially hydrogenated MOAH. These substances can cause overestimations of ≥ 3 ring MOAH fraction during GCxGC analysis due to co-elution, which is mostly avoided using the DACC approach. Furthermore, the DACC approach can help to minimize underestimations of toxicologically relevant ≥ 3 ring MOAH caused by an unavoidable loss of MOAH during epoxidation, since natural olefins, such as terpenes, predominantly elute in MDAF, which was exemplarily shown for an olive oil and a terpene reference. The presented approach can be implemented easily in existing LC-GC-FID setup for an automated and advanced screening of MOAH to lower the need for elaborate GCxGC analysis also in routine environments.

Deciphering the impact of microenvironmental factors on cuticular hydrocarbon degradation in Lucilia sericata empty Puparia: Bridging ecological and forensic entomological perspectives using machine learning models.

Blow flies (Calliphoridae) play essential ecological roles in nutrient recycling by consuming decaying organic matter. They serve as valuable bioindicators in ecosystem management and forensic entomology, with their unique feeding behavior leading to the accumulation of environmental pollutants in their cuticular hydrocarbons (CHCs), making them potential indicators of exposure history. This study focuses on CHC degradation dynamics in empty puparia of Lucilia sericata under different environmental conditions for up to 90 days. The three distinct conditions were considered: outdoor-buried, outdoor-above-ground, and indoor environments. Five predominant CHCs, n-Pentacosane (n-C25), n-Hexacosane (n-C26), n-Heptacosane (n-C27), n-Octacosane (n-C28), and n-Nonacosane (n-C29), were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The findings revealed variations in CHC concentrations over time, influenced by environmental factors, with significant differences at different time points. Correlation heatmap analysis indicated negative correlations between weathering time and certain CHCs, suggesting decreasing concentrations over time. Machine learning techniques Support Vector Machine (SVM), Multilayer Perceptron (MLP), and eXtreme Gradient Boosting (XGBoost) models explored the potential of CHCs as age indicators. SVM achieved an R-squared value of 0.991, demonstrating high accuracy in age estimation based on CHC concentrations. MLP also exhibited satisfactory performance in outdoor conditions, while SVM and MLP yielded unsatisfactory results indoors due to the lack of significant CHC variations. After comprehensive model selection and performance evaluations, it was found that the XGBoost model excelled in capturing the patterns in all three datasets. This study bridges the gap between baseline and ecological/forensic use of empty puparia, offering valuable insights into the potential of CHCs in environmental monitoring and investigations. Understanding CHCs' stability and degradation enhances blow flies' utility as bioindicators for pollutants and exposure history, benefiting environmental monitoring and forensic entomology.

Levels, spatial distributions, and provision of petroleum hydrocarbons and phthalates in sediments from Obhur lagoon, Red Sea coast of Saudi Arabia.

The levels, spatial distribution, and sources of petroleum hydrocarbons and phthalates were assessed in surface sediment samples from the urban lagoon of Obhur near Jeddah, the largest city on the Red Sea coast of Saudi Arabia. The lagoon was divided into the inner zone, middle zone, and outer zone based on its geomorphological features and developmental activities. n-Alkanes, hopane and sterane biomarkers, and unresolved complex mixture were the major petroleum hydrocarbon compounds of the total extractable organic matter. Phthalates were also measured in the sediment samples. In the three zones, n-alkanes ranged from 89.3 ± 88.5 to 103.2 ± 114.9 ng/g, whereas the hopane and sterane biomarkers varied from 69.4 ± 75.3 to 77.7 ± 69.9 ng/g and 72.5 ± 77.9-89.5 ± 82.2 ng/g, respectively. The UCM concentrations ranged from 821 ± 1119 to 1297 ± 1684 ng/g and phthalates from 37.4 ± 34.5 65 ± 68 ng/g. The primary origins of these anthropogenic hydrocarbons in the lagoon sediments were petroleum products (boat engine discharges, boat washing, lubricants, and wastewater flows) and plasticizers (plastic waste and litter). The proportions of anthropogenic hydrocarbons derived from petroleum products in the sediment's TEOM ranged from 43 ± 33 to 62 ± 15%, while the percentages for plasticizers varied from 2.9 ± 1.2 to 4.0 ± 1.6%. The presence and inputs of these contaminants from petroleum and plastic wastes in the lagoon's sediments will eventually have an impact on its habitats, including the benthic nursery and spawning areas.

Catalytic upcycling of post-consumer multilayered plastic packaging wastes for the selective production of monoaromatic hydrocarbons.

In order to obtain extended storage life of food-grade materials and better barrier properties against environmental factors, a multilayer plastic packaging (MLP) is often used. The multilayer packaging plastics are labelled as "other" (SPI#7) category, and are manufactured with a combination of barrier plastics, rigid plastics and printing surface. Owing to their complex composition and difficulty in separating the layers of MLP, its mechanical recycling is challenging. In this study, MLP wastes (MLPWs) were collected from zero-waste garbage collection center of IIT Madras, India, and thoroughly characterized to determine their composition and plastic types. MLPWs were characterized using various physico-chemical methods such as thermogravimetric/differential scanning calorimetric analysis, Fourier transform infrared spectroscopy, bomb calorimetry, and proximate and ultimate analyses. The MLPWs were mainly made up of polyethylene (PE) and polyethylene terephthalate (PET). Further, the non-catalytic and zeolite-catalyzed fast pyrolysis of these MLPWs were studied using analytical pyrolysis coupled with gas chromatograph/mass spectrometer (Py-GC/MS). The non-catalytic fast pyrolysis of MLPWs primarily produced a mixture of aliphatic and alicyclic hydrocarbons, while zeolite catalyzed fast pyrolysis resulted in the formation of mono-aromatic hydrocarbons (MAHs). The activity of HZSM-5, zeolite Y (HY) and zeolite beta (Hß) catalysts were evaluated, and the salient products were quantified. The yields of MAHs like benzene, toluene, ethylbenzene and xylene using the zeolites followed the trend: HZSM-5 (14.9 wt%) > HY (8.1 wt%) > Hß (7.8 wt%), at 650 °C. The use of HZSM-5 resulted in highest yield of MAHs, viz. 16.1 wt%, at the optimum temperature of 550 °C and MLPW-to-catalyst ratio of 1:15 (w/w). The superior activity of HZSM-5 is due to its nominal acidity and larger pore size of 4.24 nm, as compared to HY and Hß. The MAHs yield from three other types of MLPWs varied in the range of 9-16 wt%. The present study demonstrates a promising pathway for the catalytic upcycling of highly heterogeneous MLPWs in the context of circular economy.

Metagenomic analysis reveals the microbial response to petroleum contamination in oilfield soils.

The response of the microbes to total petroleum hydrocarbons (TPHs) in three types of oilfield soils was researched using metagenomic analysis. The ranges of TPH concentrations in the grassland, abandoned well, working well soils were 1.16 × 102-3.50 × 102 mg/kg, 1.14 × 103-1.62 × 104 mg/kg, and 5.57 × 103-3.33 × 104 mg/kg, respectively. The highest concentration of n-alkanes and 16 PAHs were found in the working well soil of Shengli (SL) oilfield compared with those in Nanyang (NY) and Yanchang (YC) oilfields. The abandoned well soils showed a greater extent of petroleum biodegradation than the grassland and working well soils. Α-diversity indexes based on metagenomic taxonomy showed higher microbial diversity in grassland soils, whereas petroleum-degrading microbes Actinobacteria and Proteobacteria were more abundant in working and abandoned well soils. RDA demonstrated that low moisture content (MOI) in YC oilfield inhibited the accumulation of the petroleum-degrading microbes. Synergistic networks of functional genes and Spearman's correlation analysis showed that heavy petroleum contamination (over 2.10 × 104 mg/kg) negatively correlated with the abundance of the nitrogen fixation genes nifHK, however, in grassland soils, low petroleum content facilitated the accumulation of nitrogen fixation genes. A positive correlation was observed between the abundance of petroleum-degrading genes and denitrification genes (bphAa vs. nirD, todC vs. nirS, and nahB vs. nosZ), whereas a negative correlation was observed between alkB (alkane- degrading genes) and amo (ammonia oxidation), hao (nitrification). The ecotoxicity of petroleum contamination, coupled with petroleum hydrocarbons (PH) degradation competing with nitrifiers for ammonia inhibited ammonia oxidation and nitrification, whereas PH metabolism promoted the denitrification process. Moreover, positive correlations were observed between the abundance of amo gene and MOI, as well as between the abundance of the dissimilatory nitrate reduction gene nirA and clay content. Thus, improving the soil physicochemical properties is a promising approach for decreasing nitrogen loss and alleviating petroleum contamination in oilfield soils.

Cyclopropane Hydrocarbons from the Springtail Vertagopus sarekensis─A New Class of Cuticular Lipids from Arthropods.

The epicuticle of insects is usually coated with a complex mixture of hydrocarbons, primarily straight-chain and methyl-branched alkanes and alkenes. We were interested in whether springtails (Collembola), a sister class of the insects, also use such compounds. We focused here on Vertagopus sarekensis, an abundant Isotomidae species in European high alpine regions, exhibiting coordinated group behavior and migration. This coordination, suggesting chemical communication, made the species interesting for our study on epicuticular hydrocarbons in springtails with different degrees of group behavior. We isolated a single hydrocarbon from its surface, which is the major epicuticular lipid. The structure was deduced by NMR analysis and GC/MS including derivatization. Total synthesis confirmed the structure as cis,cis-3,4,13,14-bismethylene-24-methyldotriacontane (4, sarekensane). The GC/MS analyses of some other cyclopropane hydrocarbons also synthesized showed the close similarity of both mass spectra and gas chromatographic retention indices of alkenes and cyclopropanes. Therefore, analyses of cuticular alkenes must be performed with appropriate derivatization to distinguish these two types of cuticular hydrocarbons. Sarekensane (4) is the first nonterpenoid cuticular hydrocarbon from Collembola that is biosynthesized via the fatty acid pathway, as are insect hydrocarbons, and contains unprecedented cyclopropane rings in the chain, not previously reported from arthropods.

Do distributions of diamondoid hydrocarbons accumulated in oil-contaminated fish tissues help to identify the sources of oil?

Identifying the sources of environmental oil contamination can be challenging, especially for oil in motile organisms such as fish. Lipophilic hydrocarbons from oil can bioaccumulate in fish adipose tissue and potentially provide a forensic "fingerprint" of the original oil. Herein, diamondoid hydrocarbon distributions were employed to provide such fingerprints. Indices produced from diamondoids were used to compare extracts from fish adipose tissues and the crude and fuel oils to which the fish were exposed under laboratory conditions. A suite of 20 diamondoids was found to have bioaccumulated in the dietary-exposed fish. Cross-plots of indices between fish and exposure oils were close to the ideal 1:1 relationship. Comparisons with diamondoid distributions of non-exposure oils produced overall, but not exclusively, weaker correlations. Linear Discriminatory Analysis on a combined set of 15 diamondoid and bicyclane molecular ratios was able to identify the exposure oils, so a use of both compound classes is preferable.

Comparing different methods for olefin quantification in pygas and plastic pyrolysis oils: Gas chromatography-vacuum ultraviolet detection versus comprehensive gas chromatography versus bromine number titration.

In steam cracking, upstream pyrolysis oil hydroprocessing, and in many downstream processes, olefinic content is key to assess process performance and process safety risk associated with highly exothermic reactions. When looking to plastic pyrolysis oils as a potential feedstock, as well as downstream products such as pyrolysis gasoline (pygas), these materials contain unsaturated hydrocarbons which are not present in fossil feedstocks. Pygas is a product of pyrolysis and exhibits a large number of chemical structural similarities with plastic pyrolysis oils, especially in terms of olefins structure. Quantification of the unsaturation content (olefins and di-olefins) is extremely important in industry, hence the focus of this manuscript. Detailed hydrocarbon analysis with flame ionization detection is inadequate to fully characterize the hydrocarbon composition of such samples, especially when peaks are closely eluting, or even co-eluting. In this study, the gas chromatography coupled to vacuum ultraviolet (GC-VUV) detection method previously described for the analysis of liquid hydrocarbon streams1 and plastic pyrolysis oils2 has been compared with comprehensive gas chromatography (GC × GC) and the industry standard for olefin quantification (i.e., bromine number titration). Although based on different methodologies, a correlation between the olefin content obtained from GC-VUV and the bromine number titration method is hereby presented.

Molecular responses of sponge larvae exposed to partially weathered condensate oil.

Anthropogenic inputs of petroleum hydrocarbons into the marine environment can have long lasting impacts on benthic communities. Sponges form an abundant and diverse component of benthic habitats, contributing a variety of important functional roles; however, their responses to petroleum hydrocarbons are largely unknown. This study combined a traditional ecotoxicological experimental design and endpoint with global gene expression profiling and microbial indicator species analysis to examine the effects of a water accommodated fraction (WAF) of condensate oil on a common Indo-Pacific sponge, Phyllospongia foliascens. A no significant effect concentration (N(S)EC) of 2.1 % WAF was obtained for larval settlement, while gene-specific (N(S)EC) thresholds ranged from 3.4 % to 8.8 % WAF. Significant shifts in global gene expression were identified at WAF treatments ≥20 %, with larvae exposed to 100 % WAF most responsive. Results from this study provide an example on the incorporation of non-conventional molecular and microbiological responses into ecotoxicological studies on petroleum hydrocarbons.

Fate, behaviour and microbial response of diluted bitumen and conventional crude spills in a simulated warm freshwater environment.

Diluted bitumen (DB), one of the most transported unconventional crude oils in Canada's pipelines, raises public concerns due to its potential spillage into freshwater environments. This study aimed to compare the fate and behaviour of DB versus conventional crude (CC) in a simulated warm freshwater environment. An equivalent of 10 L of either DB or CC was spilled into 1200 L of North Saskatchewan River (NSR) water containing natural NSR sediment (2.4 kg) in a mesoscale spill tank and its fate and behaviour at air/water temperatures of 18 °C/24 °C were monitored for 56 days. Oil mass distribution analysis showed that 42.3 wt % of CC and 63.6 wt% of DB resided in the oil slicks at the end of 56-day tests, consisting mainly high molecular weight (HMW) compounds (i.e., resins and asphaltenes). The lost oil contained mainly low molecular weight (LMW) compounds (i.e., light saturates and some aromatics) into the atmosphere, water column, and sediment through collective weathering processes. Notably, weathered CC emulsified with water and remained floating until the end, while the weathered DB mat started to lose its buoyancy after 24 days under quiescent conditions and resurfaced once waves were applied. Analysis of the microbial communities of water pre- and post-spills revealed the replacement of indigenous microbial communities with hydrocarbon-degrading species. Exposure to CC reduced the microbial diversity by 12%, while exposure to DB increased the diversity by 10%. During the early stages of the spill (up to Day 21), most dominant species were positively correlated with the benzene, toluene, ethylbenzene, and xylenes (BTEX) content or polycyclic aromatic hydrocarbon (PAH) content of the water column, while the dominant species at the later stages (Days 21-56) of the spill were negatively correlated with BTEX or PAH content and positively correlated with the total organic carbon (TOC) content in waters.

Effectiveness of grease interceptors in food service establishments for controlling fat, oil and grease deposition in the sewer system.

The water industry worldwide experiences numerous sewer blockages each year, partially attributed to the accumulation of fat, oil and grease (FOG). Managing this issue involves various strategies, including the requirement for installation of grease interceptors (GIs) installation. However, the claimed efficacy of commercial GIs of eliminating 99 % of FOG has been questioned for many years because FOG deposit formation occurs despite food service establishments (FSEs) using GIs, therefore detailed understanding of FOG wastewater compositions and its removal by GIs is required. This study provides an insight into the key FOG components such as FOG particle size, metals and fatty acid (FA) profile in GI influent and effluent, and within the GI, at three different FSEs. Analysis of FAs identified substantial proportions of extra-long-chain FAs in the effluents, including arachidic (C20:0), behenic (C22:0), mead (C20:3), lignoceric (C24:0), and nervonic (C24:1) acids. In contrast, the household kitchen released palmitic (C16:0), oleic (C18:1) and linoleic (C18:2) acids. It was further observed that scums effectively remove the larger FOG particles, leaving only 10 % below 75.4 µm. Notably, FSEs which employed automatic dishwashers produced up to 80.4 % of particles ≤45 µm, whereas FSEs and household kitchen which used handwash sinks generated only 36.9 % and 26.3 % of particles ≤45 µm, respectively. This study demonstrated that the commercial GIs do not remove FOG entirely but clearly demonstrated that they discharge high concentrations of FOG with extra-long FFAs which were attributed to the occurrence of microbial activity and hydrolysis of triglycerides within the GI, potentially contributing to FOG deposition.

Critical review on the formations and exposure of polycyclic aromatic hydrocarbons (PAHs) in the conventional hydrocarbon-based fuels: Prevention and control strategies.

Polycyclic aromatic hydrocarbons (PAHs) are widely present in the atmosphere and primarily originate from the incomplete burning of fossil fuels and biofuels. Exposure to PAHs leads to harmful effects on human health and the environment. Diesel engines are a major source of PAH production in the transportation sector. Various approaches have been employed to reduce PAH emissions from diesel engines, including the use of biodiesel, green gaseous fuels, exhaust gas recirculation, exhaust after-treatment, and genetically modifying biodiesel with nanoparticles. This review focuses on PAH emissions from different generations of fuels and examines the remedial control actions taken to mitigate PAH formation. The study underscores the necessity for effective regulation of emissions from diesel engines, especially in developing countries where the reliance on fossil fuels is significant. Biodiesel has shown promise in reducing PAHs and carcinogenic pollutants, with higher biodiesel concentrations resulting in lower PAH formation. Replacing diesel with biodiesel and optimizing engine operating conditions are feasible methods to reduce PAH levels in the atmosphere. The use of nanoparticles in fuel blends and higher oxygen content in combustion chambers are also considered potential strategies for pollutant reduction. Additionally, the utilization of hydrogen and ammonia as secondary fuels has been explored as promising alternatives to fossil fuels. The study highlights the importance of further research on the presence of residual PAHs in the atmosphere and the implementation of strategies to curtail vehicular emissions.

Vertical Light Non-Aqueous Phase Liquid (LNAPL) distribution by Rn prospecting in monitoring wells.

In the frame of a collaboration between the Italian National Research Council (CNR) and Mares s.r.l., a study, about the possibility of determining radon vertical distribution at different soil depths in order to trace light non-aqueous phase liquid (LNAPL) contaminations, was developed. The radon deficit technique, based on the preferential solubility of soil gas radon into non-polar fluids, such as refined hydrocarbons, has been investigated by various theoretical and applied research so far. According to international scientific literature, radon deficit can be used both for geochemical prospection of the spatial irregular NAPL dispersion and for monitoring of remediation activities. Even though it is well known that this type of pollutants can be distributed along the vertical soil profile-firstly due to their density in comparison to water density, and secondly due to fluctuations of shallow aquifers, soil pore size, aging of contamination, and so on-the vertical localization of the plume still represents a scientific challenge. In this article, a method to determine the radon vertical profile is tested and applied to assess the potential use of the radon deficit technique in the vertical detection of pollutant presence for the first time in a fuelling station. Two LNAPL-contaminated sites were selected for a pilot test. Experimental findings seem to support the use of vertical radon geochemical prospection to delimit the depth range of a LNAPL pollution directly. Systematic data collection and modeling may lead to a 3D reconstruction of the dispersion of contaminant in different soil levels.